I'd start by looking at Task Manager (ctrl+shift+esc) to see if there is a copy that is not visible but is still loaded into memory. You must look at both the Apps and Background Processes list to be sure. If you find nothing, check the file properties in File Explorer. After that I might also try opening Access first, and then open the db rather than opening the db directly if that is what you do. Do nothing except File/Save and reopen and see if the issue persists.
The more we hear silence, the more we begin to think about our value in this universe.
Paraphrase of Professor Brian Cox.